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Following primers were used to generate the W407A mutation: forward 5-CCATAGTTACTTTTCGCCATTGGAATGGAC-3 and reverse 5-GTCCATTCCAATGGCGAAAAGTAACTATGG-3, as well as forward 5-CCATAGTTACTTTGCGCCATTGGAATGGAC-3 and reverse 5-GTCCATTCCAATGGCGCAAAGTAACTATGG-3

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